Journal: iScience

Article Title: Fabrication of RADA32/Ngf_EE/MSCs composite hydrogel and its protective mechanism against radiation-induced ototoxicity

doi: 10.1016/j.isci.2026.115723

Figure Lengend Snippet: RNM composite gel mitigates radiation-induced damage in vitro (A) Viability of HEI-OC1 cells after different doses of radiation treatment, detected by CCK-8 assay. (B) Apoptosis of HEI-OC1 cells analyzed by flow cytometry after radiation exposure and subsequent transwell co-culture with RN gel, MSCs, or the RNM gel system. Data are represented as the mean ± SEM. ( N = 3, t test). (C) Distribution of γ-H2AX (red) in HEI-OC1 cells observed under a confocal microscope after radiation exposure and intervention with RN, MSCs, or RNM. Scale bars, 10 μm. (D, E, G, and H) mRNA expression levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) in mouse cochlear tissues evaluated by real-time qPCR after intratympanic injection of RN, MSCs, or RNM hydrogel following radiation exposure. Data are represented as the mean ± SEM ( N = 3, t test). (F) Intracellular ROS levels in HEI-OC1 cells monitored using the DCFH-DA fluorescent probe and flow cytometry after radiation exposure and intervention with RN, MSCs, or RNM. Data are represented as the mean ± SEM ( N = 3, t test). (I) mRNA expression levels of pro-inflammatory cytokines (IL-1β, IL-6, and TNF-α) in HEI-OC1 cells detected by real-time qPCR after radiation exposure and intervention with RN, MSCs, or RNM. Data are represented as the mean ± SEM ( N = 3, t test). Significant differences between the groups are indicated by ∗ for p < 0.05, ∗∗ for p < 0.01, ∗∗∗ for p < 0.001, and ∗∗∗∗ for p < 0.0001.

Article Snippet: Primary antibody incubation was carried out using γ-H2AX antibody (GB111841-50, Servicebio) diluted in 1% BSA at 4°C overnight.

Techniques: In Vitro, CCK-8 Assay, Flow Cytometry, Co-Culture Assay, Microscopy, Expressing, Injection

Journal: Drug Design, Development and Therapy

Article Title: The Effect of AZD5153 on Radiosensitivity in Pancreatic Cancer Cells Through ATM-chk1 Pathway

doi: 10.2147/DDDT.S568551

Figure Lengend Snippet: AZD5153 increased DNA damage in pancreatic cancer cells. ( a) The representative images of γ-H2AX foci immunofluorescence staining of PANC1 and Capan2 cells treated with 4 Gy radiation with or without AZD5153, n=6. ( b ) The protein expression of γ-H2AX in different groups was detected by western blot, β-actin was used as a loading control, n=6. Results shown are the means ± SD of 3 independent experiments with similar results for all assays. Significance was determined by Student’s t -test (** p < 0.01).

Article Snippet: The cells were fixed with 4% paraformaldehyde for 30 min, and permeabilized with 0.1% Triton-X100 cells for 15 min. After 1 h of protein blocking with 5% BSA at room temperature, the cells were incubated with anti-γ-H2AX antibodies (1:400, CST, 7631s) overnight at 4 °C.

Techniques: Immunofluorescence, Staining, Expressing, Western Blot, Control